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          Recombinant Human CD40 Ligand/TNFSF5 (Trimer)(monomeric hFc Tag)
           
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          產(chǎn)品說明書
                  
          FAQ
                  
          COA
                  
          已發(fā)表文獻
                  
           
           
          


          CD40 Ligand, also known as TNFSF, a type II membrane protein with homology to TNF, is transiently expressed on activated T cells and known to be important for B cell Ig production and for activation and differentiation of monocytes and dendritic cells. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region . It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS.  CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. 
          


           
          


          產(chǎn)品性質(zhì)
          


          
	
		
          
			
			
          別名
          
			          		
			
			
          CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
          
			          		
		
          
		
          
			
			
          Uniprot No.
          
			          		
			
			
          P29965
          
			          		
		
          
		
          
			
			
          表達區(qū)間及表達系統(tǒng)
          
			          		
			
			
          Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 Cells with monomeric hFc tag at the N-terminal. It contains Met113-Leu261.
          
			          		
		
          
		
          
			
			
          分子量
          
			          		
			
			
          Approximately 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.
          
			          		
		
          
		
          
			
			
          純度
          
			          		
			
			
          > 95% as determined by SDS-PAGE and HPLC.
          
			          		
		
          
		
          
			
			
          活性
          
			          		
			
			
          ELISA Data: Immobilized Human CD40, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 0.13μg/ml determined by ELISA.
          

			
          Blocking Data：Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
          
			          		
		
          
		
          
			
			
          內(nèi)毒素
          
			          		
			
			
          < 1.0 EU per 1μg of the protein by the LAL method.
          
			          		
		
          
		
          
			
			
          制劑
          
			          		
			
			
          Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
          
			          		
		
          
		
          
			
			
          復(fù)溶
          
			          		
			
			
          Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
          
			          		
		
          
	          

          


           
          


          運輸與保存方法
          


          冰袋運輸。-20℃至-80℃保存，一年有效期。
          


          復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復(fù)溶后，2-8°C 保存 2-7 天。
          


          建議第一次使用時分裝凍存，避免反復(fù)凍融。
          


           
          


          注意事項
          


          1. 避免反復(fù)凍融。
          


          2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
          


          3. 本產(chǎn)品僅作科研用途！
          


           
          


          產(chǎn)品數(shù)據(jù)
          


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          HB220809
          


           
          

             
           
           
           
           
           
           
           
          400-6111-883