PD-L1, also known as B7-H1, PDL1, is one of the ligands for PD-1 and plays a critical role in the regulation of T cell immunity. The PD-L1 protein is an approximately 65 kDa transmembrane glycoprotein belonging to the B7 family of immune regulatory molecules. Mature human PD-L1 protein consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain. Within the ECD, human PD-L1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. PD-L1 provides a molecular stop signal to the adaptive immune system helping to distinguish between self and foreign antigens. PD-L1 also plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development.

 

產(chǎn)品性質(zhì)

別名	CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Uniprot No.	Q9NZQ7.1
表達區(qū)間及表達系統(tǒng)	Recombinant Biotinylated Human PD-L1/B7-H1 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Phe19-Arg238.
分子量	Approximately 28.1 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
純度	> 95% as determined by SDS-PAGE and HPLC.
活性	ELISA Data：Immobilized Biotinylated Human PD-L1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA.
內(nèi)毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制劑	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
復溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

運輸與保存方法

冰袋運輸。-20℃至-80℃保存，一年有效期。

復溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復溶后，2-8°C 保存 2-7 天。

建議第一次使用時分裝凍存，避免反復凍融。

 

注意事項

1. 避免反復凍融。

2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。

3. 本產(chǎn)品僅作科研用途！

 

產(chǎn)品數(shù)據(jù)

 

 

HB220805