產(chǎn)品說(shuō)明書(shū)
FAQ
COA
已發(fā)表文獻(xiàn)
該基因是聚集在17號(hào)染色體q臂上的幾種細(xì)胞因子基因之一。趨化因子是參與免疫調(diào)節(jié)和炎癥過(guò)程的分泌蛋白的超家族。超家族根據(jù)成熟肽的N端半胱氨酸殘基的排列分為四個(gè)亞家族。這種趨化因子是CC亞家族的成員，其特征在于兩個(gè)相鄰的半胱氨酸殘基。這種細(xì)胞因子對(duì)單核細(xì)胞和嗜堿性粒細(xì)胞表現(xiàn)出趨化活性，但對(duì)嗜中性粒細(xì)胞或嗜酸性粒細(xì)胞不具有趨化活性。它與以單核細(xì)胞浸潤(rùn)為特征的疾病的發(fā)病機(jī)制有關(guān)，如牛皮癬，類(lèi)風(fēng)濕性關(guān)節(jié)炎和動(dòng)脈粥樣硬化。它與趨化因子受體CCR2和CCR4結(jié)合。
產(chǎn)品性質(zhì)
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別名 |
CCL2; MCP1; SCYA2; C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2 |
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SwissProt ID |
P13500 |
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基因ID |
6347 |
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免疫原 |
Purified recombinant fragment of human CCL2 expressed in E. Coli. |
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緩沖液 |
Purified antibody in PBS with 0.05% sodium azide. |
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分子量 |
Calculated MW: 11 kDa; Observed MW: 11 kDa |
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克隆類(lèi)型 |
Monoclonal Antibody |
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亞型 |
IgG1 |
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宿主 |
Mouse |
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物種反應(yīng) |
Human,Mouse,Monkey |
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推薦應(yīng)用 |
WB,IHC-P,ICC/IF,FC |
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純化方式 |
Affinity Chromatography |
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細(xì)胞定位 |
分泌 |
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研究領(lǐng)域 |
免疫學(xué)與炎癥，心血管 |
應(yīng)用
稀釋比 WB: 1/500-1/1000 IHC: 1/100-1/200 IF: 1/50-1/200 FC: 1/50-1/100
運(yùn)輸與保存方法
冰袋運(yùn)輸，-20℃保存。
注意事項(xiàng)
1.避免反復(fù)凍融。
2.為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
3.本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
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Immunohistochemistry |
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IHC analysis of paraffin-embedded liver cancer tissues using MCP1 antibody with DAB staining.High pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. |
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Immunofluorescence |
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IF analysis of MCP1 in HepG2 cells using MCP1 antibody. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor555 phalloidin. |
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IF analysis of MCP1 in HepG2 cells using MCP1 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor555 phalloidin. |
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Flow cytometry |
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Flow cytometry analysis of A549 stained with MCP1 antibody (green) and negative control (red). |
應(yīng)用縮寫(xiě)
WB:蛋白質(zhì)印跡法；IHC-P:石蠟切片樣本的免疫組織化學(xué)法；IHC-F:冰凍切片樣本的免疫組織化學(xué)法；ICC/IF:免疫細(xì)胞熒光； IP:免疫沉淀法；FC:流式細(xì)胞術(shù)；ChIP:染色質(zhì)免疫沉淀法；ELISA:酶聯(lián)免疫吸附測(cè)定。
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HB221020
