表達區(qū)間及表達系統(tǒng)（Source）

Recombinant Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(R167).[Uniprot | P12318-1]

分子量大小

（Molecular Weight）

The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

 活性（Activity）

SPR Data :Biotinylated Human Fc gamma RIIA (R167) , His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 2.17 μM as determined in SPR assay.

SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (R167) , His-Avi Tag with an affinity constant of 1.03 μM as determined in SPR assay

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.