ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.

 

產(chǎn)品性質(zhì)

別名	ESR1; Era; Eralpha; Estrogen receptor; Estradiol receptor; ER-alpha; Estrogen receptor 1; NR3A1; ER; ESR; ESRA; Estrogen receptor alpha
SwissProt ID	P03372
基因ID	2099
免疫原	A synthetic peptide of human Estrogen Receptor alpha.
緩沖液	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.
分子量	Calculated MW: 66 kDa; Observed MW: 66 kDa
克隆類(lèi)型	Monoclonal Antibody
宿主	Rabbit
物種反應(yīng)	Human
推薦應(yīng)用	WB, IHC-F, IHC-P, ICC/IF, IP, ChIP
純化方式	Affinity Purified

 

應(yīng)用

稀釋比 WB:1/500-1/1000; IHC:1/50-1/100; IF:1/50-1/200; IP:1/20;

 

儲(chǔ)存條件

-25~-15℃保存

 

注意事項(xiàng)

1. 避免反復(fù)凍融。

2. 本產(chǎn)品僅作科研用途。

3. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并佩戴一次性手套操作。

 

產(chǎn)品數(shù)據(jù)

Western blot

Western blot analysis of Estrogen Receptor alpha in K562, HeLa lysates using Estrogen Receptor alpha antibody.

 

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Estrogen Receptor alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

 

Immunohistochemistry

Immunocytochemistry analysis of Estrogen Receptor alpha(green) in MCF-7 using Estrogen Receptor alpha antibody, and DAPI(blue).

 

Ver.CN20231017